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. 2021 Jul 2;12:4105. doi: 10.1038/s41467-021-24352-w

Fig. 8. MCPIP3 regulates cytokine secretion via Regnase-1 and NFκB pathway.

Fig. 8

a 293T cells were co-transfected with Regnase-1, TNF-CDS with 3′ UTR or without 3′ UTR. After 48 h, TNF in supernatants were measured by ELISA (n = 3 replicate wells). b Zc3h12a expression in WT BMDMs was measured by qPCR (n = 5 mice per group). c Zc3h12a expression in IMQ-treated skin of WT mice at indicated days was measured by qPCR (n = 3 mice per group). d Degradation of Zc3h12c mRNA by Regnase-1 was measured by a luciferase reporter system. Zc3h12c 3′UTR (separated into three segments) were inserted downstream of the luciferase gene (n = 6 replicates). e Zc3h12a expression in Zc3h12c+/+/Zc3h12c−/− FL3TL-pDCs and BMDMs was measured by qPCR (n = 4 mice per group). f Nfkbiz expression in Zc3h12c+/+/Zc3h12c−/− FL3TL-pDCs was measured by qPCR (n = 4 mice per group). g Degradation of Nfkbiz mRNA by MCPIP3 was measured by a luciferase reporter system (n = 6 replicates). h–j Expression of indicated NFκB family members in Zc3h12c+/+/Zc3h12c−/− FL3TL-pDCs was measured by qPCR (n = 4 mice per group). Data are representative of three (a–e) or two (f–j) independent experiments (mean ± S.D.; two-tailed Student’s t test). Source data are provided as a Source Data file.