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. 2021 Jul 2;12:4096. doi: 10.1038/s41467-021-24332-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–a″ Katanin80, labelled using a YFP-protein trap line (green in a, a″ and single channel in a′), accumulates specifically in the secretory placodal cells and not the surrounding epidermis. a″ Katanin80-YFP foci are found in an apical-medial position in the constricting population of cells (green arrows). Cell outlines are marked by E-Cadherin (magenta in a, a″). The salivary gland placode is indicated by a white dotted line and the invagination point by an asterisk. b, c Katanin80-YFP accumulates near centrosomes and microtubules. b Kat80YFP in green localises close to centrosomes marked by Asl-mCherry in red, cell outlines in white are labelled by E-Cadherin. b′ Quantification of Katanin80-YFP accumulation near centrosomes. c Katanin80-YFP (green) can be found near microtubules labelled by staining for acetylated α-tubulin (white) in the proximity of centrosomes marked by Asl-mCherry (red). d–d′ Katanin80-YFP (green) accumulates near the microtubule-nucleating centrosomes marked by Cnn (yellow), with all centrosomes marked by Asl-mCherry (cyan) and cell outlines marked by Crumbs (magenta). Solid arrowheads indicate centrosomes exhibiting stronger Cnn and Katanin accumulation, while hollow arrowheads point towards centrosomes with less or no Cnn and Katanin staining. d′ Line scan profile through both centrosomes of a single cell to illustrate the co-enrichment of Katanin80-YFP on the Cnn-enriched centrosome. Centrosome positions are marked by Asl-mCherry. e, f Live imaging of microtubules (labeled with Jupiter-GFP, a microtubule-binding protein, in green) and centrosomes marked by Asl-mCherry (magenta) reveals microtubule release from centrosomes, two examples are shown. Arrowheads point to Jupiter foci that lose association with the centrosome over the course of the movie.