Figure panel	Number of independent experiments
1b–d	2 independent experiments
1f, g	2 independent experiments
2c	2–3 independent experiments
2d, e	Live: 8 embryos imaged on 2 different days
2f, g	3 independent experiments
3a, b	2 independent experiments
3d–d’	3 independent experiments
3e-e’	2 independent experiments
3f–f’	2 independent experiments
4a–d	3 independent experiments
4f	4 independent experiments
4g	2 independent experiments
4h, i	3 independent experiments
4j–l	2 independent experiments
5a–a″	4 independent experiments
5b	2 independent experiments
5c	3 independent experiments
5d–d’	3 independent experiments
5f	Live: 5 embryos imaged over 3 different days
6b–d	4 independent experiments
6e–g	3 independent experiments
6h–h″	>4 independent experiments
6i–k	3 independent experiments
7a–c’	>4 independent experiments
7d, e	3 independent experiments
7f–h	3 independent experiments
7i–k	Live: embryos imaged over more than 5 different days (numbers of embryos on the figure)
8a–d’	2 independent experiments
8e–g	2 independent experiments
8i, j’	2 independent experiments
Suppl.1a–c	2 independent experiments
Suppl.2a–e	2 independent experiments
Suppl.3a–c	Live: 6 embryos imaged over 3 different days
Suppl.3e–g	2 independent experiments
Suppl.3h–j	Only 1 staining (5 embryos)
Suppl.4a, b	2 independent experiments
Suppl.4c, d	2 independent experiments
Suppl.4e	2 independent experiments
Suppl.4f, g’	3 independent experiments
Suppl.4h, i’	2 independent experiments
Suppl.4j–l″	Live: embryos imaged over more 5 different days for WT (3 days for gtub OE). Number of embryos on the figure
Suppl.5a–c	3 independent experiments
Suppl.5d–f	Live: 6 embryos imaged over 3 different days
Suppl.5g–i	2 independent experiments
Suppl.5j–l	Live: embryos imaged over more than 5 different days (numbers of embryos on the figure)
Suppl.6a, b″	4 independent experiments
Suppl.6f, g	2 independent experiments
Suppl.6h–h″	2 independent experiments
Suppl.6i–k	1 experiment

p-values for pairwise comparison in Fig. 2c’