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. 2021 Jul 2;11:13771. doi: 10.1038/s41598-021-93110-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of FYCO1 tissue distribution and generation of FYCO1 knockout mice. (A) Extracts from various 4-week-old male C57BL/6J mouse tissues and MEFs (30 μg of protein) were subjected to western blot analysis with anti-FYCO1 antibody. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. This experiment was performed once using one mouse. (B) The mouse FYCO1 gene was disrupted by the insertion of a neomycin resistance gene cassette (Neo) in the first coding exon. Open and filled boxes represent coding and noncoding exons, respectively. The diphtheria toxin A gene cassette (DT-A) was inserted outside of the 3’ homologous region for negative selection. Restriction enzyme sites and probes used for Southern blot analysis are indicated. (C) Lysates from 4-week-old WT and KO mouse tissues (heart and brain) were subjected to western blot analysis with anti-FYCO1 antibody. This experiment was performed once using a mouse. (D) Lysates from 4-week-old WT and KO mouse tissues (eyes) were subjected to western blot analysis with anti-FYCO1 antibody. This experiment was performed once using a mouse.