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. 2021 Jul 2;11:13756. doi: 10.1038/s41598-021-93311-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of bacterial MV. Membrane vesicles from (a) L. rhamnosus JB-1 and (b) OMV from E. coli Nissle 1917 were enumerated by nanoparticle tracking analysis (graphs) and visualized by transmission electron microscopy (inset images). Scale bars represent 100 nm. Ribbon represents ± 1 standard error of 5 technical replicates.