Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 2;11:13731. doi: 10.1038/s41598-021-92627-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ethanol production by B. subtilis strains harboring different numbers of pdc_Z and adh_S as well as fusions of the genes. Strains NZS (Δldh, pdc_Z-adh_S), N(ZS)2 (Δldh, (pdc_Z-adh_S)₂), NZS2 (Δldh, pdc_Z-(adh_S)₂), NZ:S (Δldh, pdc_Z:adh_S), and NS:Z (Δldh, adh_S:pdc_Z) containing, respectively, one copy of each gene, two copies of each gene, one pdc_Z but two adh_S, adh_S fused to pdc_Z, and pdc_Z fused to adh_S, were cultured with an initial optical density of 0.1 (600 nm) in 2YT mediums supplemented with 60 g/L glucose and incubated at 37 °C, 180 rpm. Samples taken at 24-h intervals were analyzed for: (a) ethanol production, (b) cell growth, and (c) glucose consumption.