Fig. 5.

The scRNA profiles for stromal cell lineages in non-malignant tissues and esophageal squamous cell carcinoma.

(a) The TSNE plot and overview of the stromal cells.

(b) Feature plot of ACTA2 in stromal cells.

(c) IHC staining of α-SMA on formalin-fixed and paraffin-embedded slides for the independent biospecimens (n = 8). All replicates showed the similar results.

(d) Differences in pathway activities scored per cell by GSVA among the different stromal cell subtypes. The scores of pathways are normalized.