Fig. 7.

The scRNA profiles for myeloid cells in non-malignant tissues and esophageal squamous cell carcinoma.

(a) The TSNE plot and overview of the myeloid cells.

(b) Heatmap showing the expression of marker genes in each myeloid cell cluster.

(c) Violin plots of immune checkpoints upregulated or downregulated in myeloid cells.

(d) The TSNE plot and overview of the macrophages.

(e) Heatmap showing the expression of marker genes in each macrophage cell cluster.

(f) Differences in pathway activities scored per cell by GSVA among the different macrophage cell subtypes. The scores of pathways are normalized.

(g) Pseudotime-ordered analysis of macrophages. Macrophage cell subtypes are labeled by colors.

(h) Dynamic changes in gene expression of macrophage subtypes during the transition (divided into 3 phases), subtypes are labeled by colors (upper panel).