. 2021 Apr 11;100(8):101182. doi: 10.1016/j.psj.2021.101182

Table 4.

Effect of dietary Fe source on DMT1 and FPN1 mRNA levels in small intestinal segments of broilers at 21 d of age.¹

Fe source	DMT1, RQ²^,³					FPN1, RQ²^,³
	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value
Control	2.54^Aa	0.60^Ba	0.054^C	0.362	<0.001	1.36^Aa	0.65^B	0.047^C	0.129	<0.001
FeSO₄๒7H₂O	1.19^Ab	0.25^Bb	0.052^C	0.133	<0.001	0.98^Ab	0.32^B	0.044^C	0.062	<0.001
Fe-Met W	0.72^Ab	0.31^Bb	0.052^C	0.013	<0.001	0.89^Ab	0.41^B	0.044^C	0.063	<0.001
Fe-Pro M	0.84^Ab	0.23^Bb	0.055^C	0.106	<0.001	0.79^Ab	0.48^B	0.042^C	0.105	<0.001
Fe-Pro ES	0.79^Ab	0.33^Bb	0.051^C	0.083	<0.001	0.75^Ab	0.38^B	0.041^C	0.066	<0.001
Pooled SE	0.262	0.23	0.006			0.104	0.107	0.005
P-value	<0.001	0.015	0.979			<0.001	0.253	0.929

^a,bMeans within the same column lacking a common superscript differ (P < 0.02).

^A,B,CMeans within the same row lacking a common superscript differ (P < 0.03).

Fe-Met W = Fe-Met with a weak chelation strength (Q_f = 1.37), Fe-pro M = Fe proteinate with a moderate chelation strength (Q_f = 43.6), Fe-pro ES = Fe proteinate with a extremely strong chelation strength (Q_f = 8.59 × 10³); DMT1 = divalent metal transporter 1; FPN1 = ferroportin 1; RQ = relative quantity.

The mRNA levels were calculated as the ratio of target gene mRNA to the geometric mean of GAPDH and β-action mRNA, and RQ = 2^−ΔΔCT (CT = threshold cycle).

Data represent the means of 8 replicate cages (4 birds per cage; n = 8).