. 2021 Apr 11;100(8):101182. doi: 10.1016/j.psj.2021.101182

Table 5.

Effect of dietary Fe source on B⁰AT1 and rBAT mRNA levels in small intestinal segments of broilers at 21 d of age.¹

Fe Source	B⁰AT1, RQ²^,³					rBAT, RQ²^,³
	Duodenum³	Jejunum³	Ileum³	Pooled SE	P-value	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value
Control	1.31^A	1.29^A	0.69^B	0.14	0.012	1.09^A	0.82^B	0.36^C	0.082	<0.001
FeSO₄๒7H₂O	0.85	0.81	0.57	0.10	0.113	0.99^A	0.73^B	0.40^C	0.056	<0.001
Fe-Met W	0.99	0.93	0.58	0.13	0.075	0.94^A	0.77^A	0.34^B	0.063	<0.001
Fe-Pro M	1.12	1.01	0.85	0.13	0.382	0.93^A	0.72^B	0.44^C	0.064	<0.001
Fe-Pro ES	0.99	0.91	0.73	0.12	0.252	1.04^A	0.71^B	0.34^C	0.073	<0.001
Pooled SE	0.14	0.13	0.084			0.065	0.09	0.03
P-value	0.235	0.123	0.154			0.372	0.923	0.112

^A,B,CMeans within the same row lacking a common superscript differ (P < 0.05).

Fe-Met W = Fe-Met with a weak chelation strength (Q_f = 1.37), Fe-pro M =Fe proteinate with a moderate chelation strength (Q_f = 43.6), Fe-pro ES = Fe proteinate with a extremely strong chelation strength (Q_f = 8.59 × 10³); B⁰AT1 = b^0,+-type amino acid transporter 1; rBAT = related to b^0,+-type amino acid transporter; RQ = relative quantity.

The mRNA levels were calculated as the ratio of target gene mRNA to the geometric mean of GAPDH and β-action mRNA, and RQ = 2^−ΔΔCT (CT = threshold cycle).

Data represent the means of 8 replicate cages (4 birds per cage; n = 8).