. 2021 Apr 11;100(8):101182. doi: 10.1016/j.psj.2021.101182

Table 6.

Effect of dietary Fe source on EAAT3 and LAT1 mRNA levels in small intestinal segments of broilers at 21 d of age¹^.

	EAAT3, RQ²^,³					LAT1, RQ²^,³
Fe Source	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value	Duodenum³	Jejunum³	Ileum³	Pooled SE	Pvalue
Control	1.18	1.77	1.56	0.21	0.162	0.89^A	0.72^A	0.26^B	0.11	0.001
FeSO₄๒7H₂O	0.87^B	1.43^A	1.58^A	0.14	0.003	0.85^A	0.71^A	0.23^B	0.097	<0.001
Fe-Met W	0.94^B	1.53^A	1.59^A	0.13	0.004	1.05^A	0.62^B	0.23^C	0.12	<0.001
Fe-Pro M	0.97^C	1.37^B	1.82^A	0.12	<0.001	1.17^A	0.52^B	0.28^B	0.086	<0.001
Fe-Pro ES	1.11	1.35	1.56	0.18	0.243	1.08^A	0.51^B	0.22^B	0.14	0.001
Pooled SE	0.12	0.2	0.16			0.14	0.13	0.03
P-value	0.341	0.562	0.803			0.483	0.692	0.561

^A,B,CMeans within the same row lacking a common superscript differ (P < 0.05).

Fe-Met W = Fe-Met with a weak chelation strength (Q_f = 1.37), Fe-pro M =Fe proteinate with a moderate chelation strength (Q_f = 43.6), Fe-pro ES = Fe proteinate with a extremely strong chelation strength (Q_f = 8.59 × 10³); EAAT3 = Excitatory amino acid transporter 3; LAT1 = L-type amino transporter 1; RQ = relative quantity.

The mRNA levels were calculated as the ratio of target gene mRNA to the geometric mean of GAPDH and β-action mRNA, and RQ = 2^−ΔΔCT (CT = threshold cycle).

Data represent the means of 8 replicate cages (4 birds per cage; n = 8).