. 2021 Apr 11;100(8):101182. doi: 10.1016/j.psj.2021.101182

Table 7.

Effect of dietary Fe source on y⁺LAT1 and y⁺LAT2 mRNA levels in small intestinal segments of broilers at 21 d of age.¹

Fe Source	y⁺LAT1, RQ²^,³					y⁺LAT2, RQ²^,³
	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value	Duodenum³	Jejunum³	Ileum³	Pooled SE	P value
Control	1.07^A	0.73^B	0.34^C	0.10	<0.001	1.17^A	0.65^B	0.19^C	0.13	<0.001
FeSO₄๒7H₂O	1.19^A	0.72^B	0.40^C	0.14	0.002	1.05^A	0.71^A	0.20^B	0.14	0.001
Fe-Met W	1.03^A	0.73^AB	0.41^B	0.13	0.015	0.95^A	0.54^B	0.14^C	0.095	<0.001
Fe-Pro M	1.12^A	0.75^B	0.49^B	0.12	0.007	1.04^A	0.67^B	0.21^C	0.11	<0.001
Fe-Pro ES	1.05^A	0.79^A	0.38^B	0.12	0.002	1.08^A	0.54^B	0.16^C	0.10	<0.001
Pooled SE	0.15	0.14	0.052			0.14	0.14	0.02
P-value	0.953	0.992	0.403			0.843	0.882	0.134

^A,B,CMeans within the same row lacking a common superscript differ (P < 0.05).

Fe-Met W = Fe-Met with a weak chelation strength (Q_f = 1.37), Fe-pro M =Fe proteinate with a moderate chelation strength (Q_f = 43.6), Fe-pro ES = Fe proteinate with a extremely strong chelation strength (Q_f = 8.59 × 10³); y⁺LAT1 = y⁺ L-type amino acid transporter 1; y⁺LAT2 = y⁺ L-type amino transporter 2; RQ = relative quantity.

The mRNA levels were calculated as the ratio of target gene mRNA to the geometric mean of β-action and glyceraldehyde-3-phosphate dehydrogenase mRNA, and RQ = 2^−ΔΔCT (CT = threshold cycle).

Data represent the means of 8 replicate cages (4 birds per cage; n = 8).