Table 8.
Effect of dietary Fe source on DMT1 and FPN1 protein levels in small intestinal segments of broilers at 21 d of age.1
| Fe Source | DMT1, RQ2,3 |
FPN1, RQ2,3 | ||||
|---|---|---|---|---|---|---|
| Duodenum | Jejunum | Ileum | Duodenum | Jejunum | Ileum | |
| Control | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| FeSO4·7H2O | 1.20 | 1.10 | 1.09 | 1.00 | 1.02 | 0.91 |
| Fe-Met W | 1.30 | 1.05 | 0.92 | 0.86 | 0.95 | 0.87 |
| Fe-Pro M | 1.28 | 0.97 | 1.04 | 0.83 | 1.06 | 0.97 |
| Fe-Pro ES | 1.16 | 0.97 | 0.97 | 0.89 | 0.90 | 0.99 |
| Pooled SEM | 0.13 | 0.05 | 0.08 | 0.08 | 0.11 | 0.08 |
| P-value | 0.513 | 0.262 | 0.621 | 0.453 | 0.893 | 0.712 |
1
Fe-Met W = Fe-Met with a weak chelation strength (Qf = 1.37), Fe-pro M = Fe proteinate with a moderate chelation strength (Qf = 43.6), Fe-pro ES = Fe proteinate with a extremely strong chelation strength (Qf = 8.59 × 103). DMT1 = divalent metal transporter 1; FPN1 = ferroportin 1.
2
The protein levels were calculated as the RQ of the target gene protein to the glyceraldehyde-3-phosphate dehydrogenase protein, and the average expression level of protein in the control treatment was used as a calibrator.
3
Data represent the means of 8 replicate cages (4 birds per cage; n = 8).