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. 2021 Jun 10;24(7):102714. doi: 10.1016/j.isci.2021.102714

Figure 1.

Figure 1

Cryo-FIB-SEM provides high-resolution information of sub-cellular compartments at their native state

(A) A representative slice from an FIB-SEM acquisition of a plunge frozen mammalian cell. Contrast is most likely generated from differences in surface potential of the different materials. Nuclear pores (black arrowheads) Voxel size: 10 × 10 × 10 nm. Scale bar: 1 μm.

(B–G) Overlays of representative micrographs and organelles segmentation (left) and a slice through the same organelle (right): Golgi apparatus (B), mitochondria (C), multi-vesicular body (D), endoplasmic reticulum (E), lysosome (F), and lipid droplet (G). Scale bar: 0.5 μm.