Figure 3.

Inter- and intra-mitochondrial organization as visualized by cryo-FIB-SEM

(A) Segmentation of part of the mitochondrial network (assorted colors). Nucleus (blue), Holey carbon support film (gray).

(B) A cross section of one mitochondrion showing the white particles (upper image) and the same cross section with the segmented particles (magenta; bottom image).

(C) Histogram of the size distribution of the particles inside the mitochondrial matrix shows similarity to size distribution of amorphous calcium phosphate granules (N = 287).

(D) Box plot of the same dataset; the average volume of the granules is 1.16 x 10⁵ ± 1.22 × 10⁵ nm³.