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. 2021 Jun 9;297(1):100863. doi: 10.1016/j.jbc.2021.100863

Figure 1.

Figure 1

Whole-cell patch-clamp recordings of currents associated with 5-HT transport (A–D) and membrane capacitance changes (E, F) in the presence of 8B6 scFv (A, C, E) and of 15B8 Fab (B, D, F).A and B, representative traces elicited by rapid application of 5-HT (30 μM) onto HEK293 stably expressing GFP-tagged SERT before and after superfusion with (A) 8B6 scFv (300 nM) or (B) 15B8 Fab (300 nM) in physiological ion gradients at a holding potential of –60 mV. C, comparison of the peak current amplitudes before and after 8B6 scFv (mean ± SD: –11.8 ± 2.0 and –6.2 ± 2.0 pA, respectively, n = 5, p = 0.0001; paired t test) or 15B8 Fab (–11.0 ± 3.6 and –11.6 ± 3.6 pA, respectively, n = 9, not significant, p = 0.86; paired t test) binding to SERT. D, comparison of the steady-state current amplitudes before and after application of 8B6 scFv (mean ± SD: –5.5 ± 1.0 and –5.8 ± 1.2 pA, respectively, n = 5, p = 0.11; paired t test) or 15B8 Fab (–7.8 ± 4.5 and –8.0 ± 4.7 pA, respectively, n = 9, not significant, p = 0.09; paired t test). E and F, representative traces of the apparent reduction in the membrane capacitance of HEK293 cells stably expressing GFP-tagged SERT after superfusion with 8B6 scFv (300 nM, E) or 15B8 Fab (300 nM, F).