Figure 2.

Concentration-dependent inhibition of the peak currents through SERT by 8B6 scFv.A, representative traces depict currents induced by 30 μM 5-HT before and after superfusion for 5 s with the indicated concentrations of 8B6 scFv. B, concentration–response curve for 8B6 scFv-dependent reduction in peak current, which was normalized to the peak current amplitude recorded in the absence of 8B6 scFv (n = 4 for each concentration; error bars indicate SD). The solid curve was drawn by fitting the data to the equation for a monophasic inhibition to a residual basal level resulting in an estimated IC₅₀ = 27.9 ± 7.3 nM (mean ± SD). C, concentration–response curve for the 8B6 scFv induced change (n = 4 for each concentration; error bars indicate SD), normalized to the maximum change in the membrane capacitance upon 8B6 scFv infusion. The data were fitted to the equation for a rectangular hyperbola resulting in an EC₅₀ estimate of 15.7 ± 5.1 (mean ± SD).