Figure 4.

Metabolic model analysis and drug effect on ccRCC

(A) Venn diagrams showing the overlaps of reactions, metabolites or genes between subtypes in the TCGA cohort. The reactions, metabolites, and genes are generated from reconstructed subtype-specific GEMs.

(B) Western blot showing the protein level of SOAT1 was decreased by the treatment of mitotane in Caki-1.

(C) Bar chart showing the cell viability was decreased by mitotane in Caki-1. ∗represents t test p < 0.05. Data are represented as mean ± SEM.

(D) Flow cytometric analysis showing the cell cycle G2/M was arrested by mitotane in Caki-1.