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. 2021 Jun 28;13:5077–5089. doi: 10.2147/CMAR.S276964

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© 2021 Yu et al.

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CircASXL1 bound to miR-206 in A549 and H1299 cells. (A) RIP assay was performed to explain whether circASXL1 could form silence-inducing complexes with miRNA in A549 and H1299 cells. (B) RNA pull-down assay was employed to determine the miRNAs associated with circASXL1. (C) CircBank online database was used to predict the binding sites between miR-206 and circASXL1. (D) MiR-206 expression was detected by qRT-PCR after miR-206 mimic transfection. (E) Dual-luciferase reporter assay was carried out to detect luciferase activities in A549 and H1299 cells. (F) The effect of circASXL1 knockdown on miR-206 expression was revealed by qRT-PCR. (G and H) MiR-206 expression was determined by qRT-PCR in NSCLC tissues, NSCLC normal tissues, BEAS-2B cells, A549 cells and H1299 cells. (I) qRT-PCR was utilized to detect miR-206 expression in A549 and H1299 cells after normoxia or hypoxia treatment. *P < 0.05 and **P < 0.01.