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. Author manuscript; available in PMC: 2021 Jul 3.
Published in final edited form as: Biochemistry. 2019 Dec 27;59(4):471–478. doi: 10.1021/acs.biochem.9b00952

Figure 3:

Figure 3:

h-Amylin and p vole-amylin p.vole amylin form amyloid. (A) Thioflavin-T assays of amyloid formation conducted for wild type h-amylin (Blue) and p. vole amylin (Orange) in PBS buffer (pH 7.4, 10 mM with 140 mM KCl). The vertical axis was normalized to 100 for both peptides. Representative curves are shown and non-normalized data is shown in the supporting information. (B) TEM images recorded for samples collected at the end of the kinetic experiments: wild type h-amylin (Blue) and p. vole amylin (Orange). Scale bars represent 100 nm. (C) Thioflavin-T assays of amyloid formation conducted with wild type h-amylin (Blue) and p. vole amylin (Orange) in Tris buffer (pH 7.4, 20 mM) representative curves are shown. (D) TEM images recorded for samples collected at the end of the kinetic experiments: wild type h-amylin (Blue) and p. vole amylin (Orange). Scale bars represent 100 nm.