Figure 4.

Representative images of step-by-step coverslip mounting for FISH probe hybridization

From left to right, 10 μL of probe mixture is applied to the slide with the mounted tissue, immediately, an appropriated size coverslip is applied. Once no bubbles are present, the coverslip is sealed with rubber cement. Ensuring a proper sealing is key to avoid evaporation. After hybridization using the ThermoBrite denaturation-hybridization program (not shown), the rubber cement seal is carefully removed by pulling one of the corners of the seal with forceps.