Figure 5.

Nuclei recognition by FISH: Selecting and enumerating cells within the selected target area

(A–C) Exemplary of three representative areas of tissue with a good nuclear distribution. Individual nuclei can be distinguished and show a strong FISH signal together with weak or no fluorescence background. Only the tumor nuclei with intact nuclear membrane, that do not overlap are used for the analysis. Images were captured using a Leica DM5500B fluorescence microscope with a 100× objective running the Cytovision v7.4 software. (A–C) White dashed line highlights single nuclei that meet the described criteria; scale bars, 10 μm.