Figure 6.

Pepsin treatment calibration

Representative pictures were serial sections of FFPE tissues were incubated at different time points with ready to use pepsin to test the correct time for digestion. Incubations were performed at (A) 30 min, (B) 45 min, (C) 60 min and (D) 120 min (only Dapi channel are shown) before probe hybridization. The total time of wash buffer incubation and hybridization was equal for all conditions. Images were captured using a Leica DM5500B fluorescence microscope with a 100× objective running the Cytovision v7.4 software. (A–D) scale bars, 10 μm.