Reagent	Final concentration	Amount
10× Nick Translation buffer	1×	5 uL
0.1 mM dNTP mix (dATP/dGTP/dCTP)	0.02 mM	10 uL
0.1 mM dTTP	0.01 mM	5 uL
0.1 mM Fluorescence labeled dUTP	0.01 mM	5 uL
Extracted DNA	20 ug/mL	1 ug
Nick Tranlation enzyme (DNAse I)	n/a	10 uL
Nuclease-free water	n/a	Up to 50 uL
Total	n/a	50 uL