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. 2021 May 23;297(1):100818. doi: 10.1016/j.jbc.2021.100818

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

proBACE1 interacts with proIR. HA-BACE1 and FLAG-IR overexpressed in HEK293 cells were detected by immunoblot (IB) using anti-HA, anti-BACE1 prodomain (PRO), and anti-FLAG antibodies in (A) cell lysates and (B) after immunoprecipitation (IP) of HA-BACE1 or FLAG-IR using the corresponding anti-tag antibodies. C, HA-BACE1 was immunoprecipitated (IP: HA) from lysates of cells expressing HA-BACE1 alone or with furin (FUR) then BACE1 and proBACE1 were detected. D, HA-BACE1 and/or FLAG-BACE1 were expressed as indicated, then BACE1 was detected in cell lysates and after FLAG-BACE1 immunoprecipitation (IP: FLAG); right panel: long exposure of the blot. E, HA-BACE1 and/or IR were expressed as indicated, then BACE1 and IR were detected in cell lysates and after HA-BACE1 immunoprecipitation. Positions of IR precursor (proIR), BACE1 dimer (d), proBACE1 (p), mature BACE1 (m), and heavy chain immunoglobulin (∗) are indicated.