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. 2021 May 23;297(1):100818. doi: 10.1016/j.jbc.2021.100818

Figure 6.

Figure 6

O-GlcNacylation, ubiquitination, and ER stress are increased in livers of db/db mice.A, O-GlcNacylation (O-GlcNac), total ubiquitination (Ubi), and β-actin (loading control) were detected by immunoblot in lysates of liver from standard-fat diet–fed mice (SFD) and high-fat diet–fed mice (HFD); a densitometric analysis of the signals is shown (lower panel). B, O-GlcNacylation, total ubiquitination, proBACE1, GRP78, and β-actin were detected in lysates of liver from db/+ and db/db mice; a densitometric analysis of the signals is shown (lower panel). C, mRNA levels of the indicated ER stress markers were measured by RT-PCR, in livers from SDF- and HFD-fed mice. D, BACE1 was measured by ELISA in lysates of liver from db/+ and db/db mice (same amount of protein was used). Values are expressed as fold over the means of the control mice. Data are means ± SD. Statistical analyses were made using Mann–Whitney (A, B, and D) or t test (C): ∗p < 0.05, ∗∗∗p < 0.001.