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DNA duplexes used in the current study on the Egr-1 zinc-finger protein. (A) DNA duplexes used for kinetic measurements. The solid lines indicate chemically synthesized nucleotides, whereas the dotted lines indicate enzymatically extended nucleotides. (B) DNA duplexes used for affinity measurements. (C) PAGE of the fluorescent DNA duplexes, which were visualized via fluorescence from the covalently attached FAM or TAMRA.
