Table I.
Kinetic parameters for translocation of the Egr-1 zinc-finger protein on nonspecific DNA sites determined from the stopped-flow fluorescence data.
| Parameters | Values and uncertainties a) |
|---|---|
| - Sliding - | |
| ksl,N (s−1) b) | (2.1 ± 0.1) × 105 |
| D1 (μm2 s−1) c) | (2.4 ± 0.1) × 10−2 |
| - Intersegment transfer - | |
| kit,N (M−1 s−1) d) | (1.0 ± 0.1) × 106 |
| - Dissociation - | |
| koff,N (s−1) f) | 10 ± 4 |
| kout (s−1) g) | 83 ± 3 |
| - Association - | |
| kon,N (M−1 s−1) h) | (2.3 ± 0.8) × 106 |
| - Effective sliding length - | |
| λ (bp) i) | 50 ± 2 |
a)
Uncertainties in reported values were estimated with a 68% confidence interval in fitting.
b)
From the data shown in Figure 7.
c)
From ksl,N along with Eq. 15.
d)
From the data shown in Figure 8.
f)
From the data shown in Figure 8.
g)
From the data shown in Figure 7. This actually corresponds to kout,CP because of the strong presence of intersegment transfer.
h)
From koff,N and Kd,N.
i)
From ksl,N and kout along with Eq. 21.