Fig. 3.

The registration procedure; A flowchart of the process to register histopathology to in-vivo PET/MRI data. A) The histopathology slice is rigidly registered to its corresponding ex-vivo MRI before they are registered with a 2D affine registration. B) A 3D rigid registration is applied to move the ex-vivo MRI to the in-vivo MRI, by using the pre-defined in-vivo prostatic volume and a mask of the ex-vivo MRI volume. C) The contours of the ex-vivo MRI were registered to the defined in-vivo MRI volume to correct for tissue distortions occurring when the specimen was in the prostate mold. D) Shows an example of a patient with large extraprostatic margins after surgery. The green ROI represents the ex-vivo contour and the white ROI represents the delineated prostatic volume from the transaxial in-vivo MRI. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)