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. 2021 Jul 2;12:379. doi: 10.1186/s13287-021-02460-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of extracellular vesicles derived from ESC and MEF. a Western blot analysis of the marker of EVs, Alix, and TSG101, in ESC-EVs, MEF-EVs, ESC, and MEF. b Transmission electron microscope (TEM) image of MEF-EVs and ESC-EVs. Scale bar represents 50 nm. c Nanoparticle tracking analysis (NTA) indicated the particle size distribution of MEF-EVs and ESC-EVs. d Internalization of ESC-EVs and MEF-EVs were analyzed by immunofluorescence detection. DiI-labeled EVs (red) were detected in the HK-2 cells and HUVECs which were labeled by anti-Tubulin (green). Scale bar represents 50 μm