Table 2.
Primers used for quantitative real-time polymerase chain reaction to detect bacterial numbers
| Targeted bacterial group | Amplicon size (bp) | Primer sequence (5’–3’)1 | Annealing temperature (°C) |
|---|---|---|---|
| Total eubacteria | 200 | F:CGG(C/T)CCAGACTCCTACGGG R:TTACCGCGGCTGCTGGCAC |
58 |
| Lactobacillus spp. | 341 | F:AGCAGTAGGGAATCTTCCA R:CACCGCTACACATGGAG |
62 |
| Enterobacterium spp. | .195 | F:CATTGACGTTACCCGCAGAAGAAGC R:CTCTACGAGACTCAAGCTTGC |
60 |
| Bifidobacteria spp. | 243 | F:TCGCGTC(C/T)GGTGTGAAAG R:CCACATCCAGC(A/G)TCCAC |
58 |
| Enterococcus spp. | 144 | F:CCCTTATTGTTAGTTGCCATCATT R:ACTCGTTGTACTTCCCATTGT |
60 |
1
F = forward; R = reverse.