Table 3.
Sequences of the oligonucleotide primers used for quantitative real-time polymerase chain reaction
| Genes | GenBank number | Primer sequence (5’–3’)1 | Product length (bp) |
|---|---|---|---|
| Interleukin-1α (IL-1α) | NM_214029 | F:CTGAAGAAGAGACGGTTGAG R:GCACTGGTGGTTGATGAC |
162 |
| Interleukin-1β (IL-1β) | NM_214055 | F:GGCCGCCAAGATATAACTGA R:GGACCTCTGGGTATGGCTTTC |
70 |
| Interleukin-8 (IL-8) | NM_213867 | F:CTGGCTGTTGCCTTCTTG R:GTCCACTCTCAATCACTCTC |
163 |
| β-Actin | AY550069 | F:ATGCTTCTAGGCGGACTGT R:CCATCCAACCG ACTGCT |
211 |
| Toll-like receptors 4 (TLR-4) | NM_001293316.1 | F:CAGATAAGCGAGGCCGTCATT R:TTGCAGCCCACAAAAAGCA |
113 |
| Myeloid differentiation primary response 88 (MyD88) | NM_001099923.1 | F:TGGTAGTGGTTGTCTCTGATGCAA R:TGGAGAGAGGCTGAGTGCAA |
80 |
1
F = forward; R = reverse.