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. 2021 May 10;65:10.29219/fnr.v65.7577. doi: 10.29219/fnr.v65.7577

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© 2021 Cui Lin et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

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Fig. 4 — Effects of sesamol on lipid accumulation and expressions of beige-specific genes and proteins in mature 3T3-L1 adipocytes. The mature 3T3-L1 adipocytes were treated with different concentrations of sesamol (0, 12.5, 25, and 50 μΜ) for 48 h. (A) Mature 3T3-L1 adipocytes were stained with oil red O to observe lipid droplets at 200 × magnification. Scale bar = 200 μm. (B) RT-PCR was used to detect the levels of beige-specific genes. (C) Immunoblotting was used to detect protein expression of UCP1 and densitometric determinations. All values are presented as mean ± SD (n = 3). β-actin and histone were protein loading control. *P < 0.05 versus control group (0 μM sesamol).