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. 2021 May 10;65:10.29219/fnr.v65.7577. doi: 10.29219/fnr.v65.7577

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© 2021 Cui Lin et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

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Fig. 9 — Effects of SR 59230A on the expressions of beige-specific genes and proteins in mature 3T3-L1 adipocytes treated with sesamol. The mature 3T3-L1 adipocytes were pretreated with 10 μM SR 59230A for 2 h prior to sesamol (50 μM) treatment. RT-PCR was used to detect the genes level and immunoblotting was used to detect protein expression. (A) β3-AR levels and densitometric determinations. (B) PKA-C, p-PKA substrate levels, and densitometric determinations. (C) Mature 3T3-L1 adipocytes were stained with oil red O to observe lipid droplets at 200 × magnification. (D) The levels of beige-specific genes. (E) Protein expression of UCP1 and densitometric determinations. All values are presented as mean ± SD (n = 3). β-actin and histone were protein loading control. *P < 0.05 versus the control group; #P < 0.05 versus the sesamol group.