RFOs are hydrolyzed in fruits. A, Sugar content measured by ion chromatography in watermelon stem (S) and carpopodium (C) vascular exudate. B, Fruit and mesocarp sugar content in wild and cultivated accessions. Samples collected from center fruit and mesocarp were measured by ion chromatography after freeze drying. Average values ± standard deviation (sd) for n = 3 independent biological replicates are shown. **t-test significant at P < 0.01. C, Reciprocal grafting between wild and cultivated fruits (CVs). The grafting of cultivar 97103 fruit to stem of wild accession PI 296341 (left), and the grafting of wild accession PI 296341 fruit to stem of cultivar 97103 (right). The harvested fruits after grafting were shown in the middle. The grafting site between two accessions is shown as “+”. Bars = 5 cm. D, Sugar content in reciprocal grafted fruits. NS, no significant difference. E, Raf content in the non-sweet wild ancestors and cultivated sweet watermelons measured by FW. Each dot represents one accession, and the mean for n = 3 independent biological replicates for each accession is shown.