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. 2021 Feb 11;33(5):1554–1573. doi: 10.1093/plcell/koab055

Figure 3.

Figure 3

ClAGA2 enhances sugar partition in watermelon. A, ClAGA2 overexpression accelerates fruit coloration. Two independent fruits at 34 DAP for each are shown. Bar = 10 cm. B, Relative mRNA levels of ClAGA2 in OE lines and WT at 34 DAP. Means ± sd of n = 3 independent replicates are shown. **t-test significant at P < 0.01 for mRNA levels of ClAGA2 OE lines compared to WT. C and D, ClAGA2 overexpression increased alpha-galactosidase activity (C) and accelerated Raf hydrolyzation and sugar accumulation (D). Means ± sd of n = 3 independent replicates are shown. *t-test significant at P < 0.05 and **t-test significant at P < 0.01 for sugar content of ClAGA2 OE lines compared to WT. E, Two lines of claga2 mutant plants at 18 DAP. F, Raf content in the claga2 mutants, WT and PI 296341. Means ± sd of n = 2 independent replicates are shown. *t-test significant at P < 0.05 and **t-test significant at P < 0.01. G, Fruit Glc, Fru, and Suc contents in claga2 mutants at 30 DAP. Average values ± sd for n = 3 independent biological replicates are shown. **t-test significant at P < 0.01. H, Biomass measured on FW and DW of the claga2 mutants and WT. *t-test significant at P < 0.05, **t-test significant at P < 0.01. Means ± sd of n = 3 independent replicates are shown. P-values were calculated by two-tailed, unpaired t-tests. I, Germination of WT, claga2 mutant, and wild species PI 296341 seeds incubated under dark conditions at 28°C for 80 h. Bars = 2 cm. J, Seed germination rates of claga2 mutants and WT. Means ± sd of n = 3 independent replicates are shown.