Figure 4.
FaRIF regulates ABA biosynthesis and response. A, Expression of ABA biosynthetic (FaNCED3 and FaZEP) and -responsive (FaHVA22 and FaSnRK2.6) genes in control and 35Spro:RIF-RNAi white (W) and red (R) receptacles. B, ABA content (ng/FW) in control (C) and 35Spro:RIF-RNAi red receptacles. Data are means ± se of three biological replicates, analyzed by Student’s t test (*P < 0.005; **P < 0.0005). C, Ripening progression in 35Spro:RIF-RNAi receptacles 7 days after mock-infiltration with 2% ethanol or 100 μM ABA. Representative pictures of a single fruit for each 35Spro:RIF-RNAi line in each condition out of three biological replicates are shown. Fruit size cannot be directly compared across panels.