Figure 3.

TaBCAT1 expression is elevated early during infection with Pst and Blumeria graminis f. sp. tritici (Bgt). A, A total of 37 transcripts annotated with GO terms linked to BCAA biosynthetic processes were identified across co-expression clusters, with the highest enrichment in cluster VII, followed by cluster XII. B, Gene expression analysis of wheat samples infected with Pst and Blumeria graminis f. sp. tritici (Bgt) revealed three gene homeologs (red highlight) uniquely upregulated at 24 hpi and 48 hpi, respectively. C, These three gene homeologs (TaBCAT1-A, TaBCAT1-B, and TaBCAT-D) were upregulated early during infection with Pst and Bgt and also during abiotic stress. Expression levels for the 37 transcripts were assessed in publicly available wheat RNA-seq data. mo, mock; fu, Fusarium; st, Septoria tritici; pm, powdery mildew (Bgt); sr, stripe rust (Pst); hs, heat stress; ds, drought stress; h, hour; d, day; n, number of replicates. Error bars represent standard errors