Figure 11.

The LSU-HMW complex is not affected by MRL1 absence in the mrl1;ΔRBCS;5′UTRpsaA:rbcL strain. (A) Scheme of the 5′UTR psaA:rbcL chimeric construct. (B) Native immunoblot using the Rubisco antibody in order to follow the migration pattern of the LSU-HMW repressor complex using soluble proteins extracted from strains expressing LSU from the 5′UTRpsaA:rbcL chloroplast transgene in an RBCS mutant, in a MRL1 WT background (ΔRBCS; 5′UTRpsaA:rbcL) or mutant background (mrl1; ΔRBCS; 5′UTRpsaA:rbcL V17 and V23). WT diluted extract, as well as extracts from the ΔrbcL and ΔRBCS strains, were included as controls. LSU oligomers are depicted by the same symbols used in Figure 5.