Figure 2.
Swapping rbcL 5′ UTR regulatory sequence impairs the CES regulation. (A) Upper panel: Photosynthetic growth phenotypes of 5′UTRpsaA:rbcL strains defective or not for Rubisco SSU, and accumulation of the corresponding Rubisco subunits tested by western blot analysis. Lower panel: In ΔRBCS;5′UTRpsaA:rbcL, LSU is accumulating to higher levels than in ΔRBCS. TAP stands for TAP medium, MIN is an acetate-free, phototrophy-selective medium. (B) 14C pulse labeling experiment showing LSU synthesis rate in 5′UTRpsaA:rbcL-background with and without small subunit compared with wild-type, ΔrbcL and ΔRBCS strains. The dashed line marks the position where two irrelevant lanes were removed.