Figure 4.

CES regulation does not occur in the absence of LSU accumulation. (A)¹⁴C labeling experiment showing synthesis rates of chloroplast proteins in WT, ΔrbcL, ΔRBCS, LSU_tr transformants (1-3), and ΔRBCS;LSU_tr (1 and 4) strains. Migration of full-length and truncated LSU is indicated on the left. The dashed line marks the position where two irrelevant lanes were removed. (B) Immunoblot depicting LSU and cyt f accumulation in representative transformants carrying both the 5′UTRrbcL:petA reporter gene and a truncation within the rbcL gene, associated or not to the ΔRBCS mutation, in comparison to the wild-type and ΔRBCS;5′UTRrbcL:petA strains. Ponceau stain and PsaD accumulation are shown as loading controls.