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. 2021 Feb 9;33(5):1748–1770. doi: 10.1093/plcell/koab048

Figure 9.

Figure 9

Performance assays show reduced growth of M. sexta fed on transformed lines impaired in HGL-DTG glycosylation. A, Mass of M. sexta larvae feeding on leaf disk material of four stably transformed plants impaired in glucosylation (IRugt74p5, IRugt74p3/ugt74p5) and rhamnosylation (IRugt91t1) of HGL-DTGs as well as the formation of their precursor geranylgeranyl diphosphate (IRggpps; average ± se; n = 24–30). Larvae grow significantly larger on IRggpps (P = 0.005) and are significantly smaller on IRugt74p3/ugt74p5 (P = 0.005) and IRugt74p5 (P = 0.006) by day 6, as determined by Mann–Whitney–Wilcox pairwise tests. For clarity, significance is only shown for day 12: *P < 0.05, **P < 0.01, ***P < 0.001. B, Mass of leaf disks from transgenic plants consumed by caterpillars (average ± se; n = 26–30 leaf disks with one larva). Larvae fed IRugt74p5, IRugt74p3/ugt74p5, and IRugt91t1 consumed significantly less leaf disk material than larvae fed WT tissue, as determined by Mann-Whitney-Wilcox Pairwise Tests. *P < 0.05, **P < 0.01, ***P < 0.001.