Figure 2.

Expression of SIRT1 in human HCC cell lines and Nanog^Pos liver CSCs. (A) The expression of SIRT1 were determined in nine HCC cell lines by Western blot. (B) Pattern diagram of Lv-PNanog-GFP lentiviral reporter system, in which Nanog promoter controlled the expression of GFP. (C) HCC cell lines were infected with Lv‐P_Nanog‐GFP and GFP-positive and negative cells were sorted by flow cytometer. (D) qPCR assay was used to detect stem cell associated markers of sorted Nanog^Pos CSCs and Nanog^Neg cells. (E) Western blotting for the detecting SIRT1 expression of sorted liver CSCs and non-CSCs. (F) Quantitation of protein levels was on the right panel. (G) Expression levels of SIRT1 were detected by immunofluorescence staining in liver CSCs and liver non-CSCs derived from HCC cell lines. All values presented as mean ± S.D.*p < 0.05, **p < 0.01. Scale bar, 100 µm.