Table 3.
Expression of Stem Cell Markers in Mlh1/1638N COL-Cl1 Cells
| Phenotype | 5-FU(µM) | Stem cell Marker Expression | |||
|---|---|---|---|---|---|
| TS a | CD44 b | CD133 b | c-Myc b | ||
| 5-FU-S | 0.5 | 2.5±0.5 | 2.7±0.3 | 2.3±0.3 | 2.2±0.2 |
| 5-FU-R | 0.5 | 24.0±4.0 | 16.6±6.6 | 14.3±3.1 | 8.5±1.9 |
| X2 | 8.49 | ||||
| P | <0.010 | <0.010 | <0.010 | <0.025 | |
| Relative to 5-FU-S | +8.6X | +5.1X | +5.2X | +2.9X | |
Notes: aTS number determined at day 14 post-seeding. Mean ± SD, n=3 per treatment group. Data analyzed by X2 test. bCD44, CD133 and c-Myc determined at day 3 post-seeding and expressed as log mean FU ± SD, n=3 per treatment group. Data analyzed by two sample Student’s t test. Abbreviations: 5-FU, 5-fluro-uracil; TS, tumor spheroid; CD, cluster of differentiation; C-Myc, cellular Myc; 5-FU-S, 5-FU-sensitive; 5-FU-R, 5-FU-resistant; X2, Chi square; SD, standard deviation; X, fold change.