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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Vet J. 2021 Apr 14;273:105676. doi: 10.1016/j.tvjl.2021.105676

Fig. 2.

Fig. 2.

Comparative immunoblot analysis of outer surface protein (Osp) C antibody specificity. Twenty-three different OspC type proteins derived from North American or European Lyme disease isolates (as indicated) were generated as His-tagged recombinant proteins and purified using Fast Protein Liquid Chromatography. The recombinant proteins were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. One gel was stained to visualize the proteins and others were immunoblotted. The blots were screened with pooled sera collected from three dogs within each vaccine group 2 weeks after the second vaccine dose on Day 35. The blots were imaged together for the same amount of time (148 s). The images were cropped to generate the figure.