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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Mol Cancer Res. 2021 Mar 22;19(7):1156–1167. doi: 10.1158/1541-7786.MCR-20-0867

Figure 1. Identification of AP-2α target genes.

Figure 1.

A. RNA sequencing of the parental colon cancer cell line HCT116 was compared to sequencing of three individual AP-2α knockout clones, yielding 131 genes whose expression was either significantly increased (red) or decreased (blue) in all three knockout clones. Gene expression changes were confirmed for select genes at the protein level using western blot (right panel). B. ChIP-seq analysis of the 131 commonly altered genes from by RNA-seq identified 37 as primary targets of AP-2α with select representation of peaks (C). D. Ingenuity Pathway Analysis identified three primary altered pathways based on gene expression after loss of AP-2α.