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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Mol Cell. 2021 May 7;81(13):2693–2704.e12. doi: 10.1016/j.molcel.2021.04.013

Figure 2. WNK1 is an EMC assembly factor.

Figure 2.

(A) HeLa EMC2-GFP CRISPR knock-in cells, in which EMC2 is expressed from its endogenous promoter, were transduced to stably express either a scrambled control or WNK1 shRNA and then analyzed for GFP fluorescence by flow cytometry. (B) HEK293T cell lines stably expressing EMC4-GFP, RFP-squalene synthase (SQS; a tail-anchored EMC substrate) and RFP-VAMP2 (a tail-anchored WRB-CAML substrate) or an unfused control (GFP-2A-RFP) were treated with the indicated siRNAs and analyzed by flow cytometry.