Figure 1. Perinatal hGPC grafts myelinate the congenitally unmyelinated shiverer brain.
A. A 1-year-old shiverer mouse, transplanted at birth with 3 × 105 human glial progenitor cells, stained for myelin basic protein (MBP, green). B. higher power view of myelinated human oligodendrocytes in the corpus callosum of a 12 week-old transplanted shiverer (MBP, green, human nuclei, red). C. By 9 months, essentially all transcallosal axons have myelinated (mouse axons in red, stained for neurofilament; human MBP, green). D. Human GPC-derived oligodendrocytes normalized nodal architecture at nodes of Ranvier, here in the cervical spinal cord of an adult, neonatally-engrafted shiverer (Caspr2, green; Contactin, blue; ßIV-spectrin, red). E, hGPC-derived oligodendrocytes produced ultrastructurally normal myelin; corpus callosum, 12 wks. Scales: A, 1 mm; B, 50 μm; C, 10 μm; D, 20 μm; E, 1 μm. Adapted from (Windrem et al., 2008).