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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Semin Cell Dev Biol. 2021 Jan 4;116:62–70. doi: 10.1016/j.semcdb.2020.12.004

Figure 6. Manufacturing hGPCs from hESCs.

Figure 6

The first part of the GMP manufacturing process is focuses on initiation from the hESC master cell bank (MCB) on Day -20, followed by hESC expansion, EB formation, and neural induction with neurosphere formation by day 26, and neural stem cell expansion through day 36. Glial induction starts on Day 36, with in-process testing at days 100, 135, and 160, and hGPC recovery with cryopreservation at 160 days. Methods as described (Wang et al., 2013), as adapted for GMP compliance. From Chandler-Militello et al., unpublished.