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. 2021 Jun 25;148(12):dev190793. doi: 10.1242/dev.190793

Fig. 5.

Fig. 5.

Regulation of eIF4E and p-eIF4E. (A) Western blots of eIF4E, p-eIF4E and actin expression in hybrid embryos (C57BL/6×CBA/He) at 16 and 22 h post-HCG. (B,C) Analysis of eIF4E and p-eIF4E expression changes relative to actin in hybrid embryos (C57BL/6×CBA/He) at 16 and 22 h post-HCG (B) and in hybrid embryos (C57BL/6×CBA/He) 16 h post-hCG treated for 4 h with either 10 µM SB203580 or 25 µM staurosporine (C). Data are representative of three independent replicates and each treatment had 50 embryos; *P<0.05, **P<0.01, compared with vehicle (univariate analysis of variance). (D) Whole-session immunolocalized images of eIF4E in hybrid embryos (C57BL/6×CBA/He) at 16 h post-hCG treated for 4 h with 10 µM SB203580 compared with vehicle. Data are representative of three independent replicates and each treatment group contained ten embryos. Scale bar: 10 µm.